Purine deoxyribonucleoside phosphorylase
Purine-nucleoside phosphorylase
CAS: 9030-21-1
Molecular Formula: C10H12N4O4
Purine deoxyribonucleoside phosphorylase - Names and Identifiers
Purine deoxyribonucleoside phosphorylase - Physico-chemical Properties
| Molecular Formula | C10H12N4O4 |
| Molar Mass | 252.22668 |
| Appearance | White freeze-dried powder. |
| Storage Condition | -20°C |
| MDL | MFCD00131739 |
| Use | It can decompose nucleosides into bases and pentose-1-phosphate, and the reaction is reversible. Purine nucleoside phosphorylase is very important in purine nucleotide remediation anabolism. The lack of purine nucleoside phosphorylase in organisms will lead to damage to T cell function. |
Purine deoxyribonucleoside phosphorylase - Risk and Safety
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| HS Code | 35079090 |
Purine deoxyribonucleoside phosphorylase - Vitality
100～200units/mg protein
Last Update：2023-08-16 21:32:38
Purine deoxyribonucleoside phosphorylase - Definition of enzyme activity
One unit will cause the phosphorolysis of one micromole of inosine to hypoxanthine and ribose 1-phosphate per min at pH 7.7 at 37℃.
Last Update：2023-08-16 21:32:38
Purine deoxyribonucleoside phosphorylase - Isoelectric point
PH 6.0
Last Update：2023-08-16 21:32:38
Purine deoxyribonucleoside phosphorylase - Inhibitors
Ag+，Hg2+
Last Update：2023-08-16 21:32:38
Purine deoxyribonucleoside phosphorylase - Thermal stability
30～70℃
Last Update：2023-08-16 21:32:38
Purine deoxyribonucleoside phosphorylase - PH stability
5.0～11.0
Last Update：2023-08-16 21:32:38
Purine deoxyribonucleoside phosphorylase - Method of use
When measuring the activity, the powder was dissolved in 50mM,PH7.7 potassium phosphate buffer solution. Most of the bacterial PNP is hexamer, the molecular weight of the subunit is 25kDa, the substrate specificity is not strong, and can accept adenosine, guanosine and inosine as substrates; mammals and some microorganisms (Bacillus cereus, Bacillus stearrothmolophushth 6-2)PNP is a trimer, subunit molecular weight of 30~32kDa, usually only accept inosine, guanosine as substrate; Some microorganisms, such as Escherichia coli, bacillus subtilis, Bacillus stearothermophilus, there will be two kinds of polymer PNP.
Last Update：2023-08-16 21:32:38
Purine deoxyribonucleoside phosphorylase - Reference Information
| Application | Purine nucleoside phosphorylase is abbreviated as PNP or PNPase, which is one of the key enzymes in purine remedial synthesis and synthesis pathways and is widely found in prokaryotes and eukaryotes. |
Last Update：2024-04-09 21:53:21
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Mobile: 18021002903
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